Observation of GFP-tagged Sphingomonas sp. SaMR12 colonization pattern with a laser scanning confocal microscope (LSCM). SaMR12 bacteria were cultured in LB medium and inoculated into plants. Roots cut off at different sampling times, (a) 2 h, (b) 12 h, (c) 24 h, and (d) 4 d and (e) a root section and (f) a stem section of Brassica juncea (l.) Czern. were captured accordingly. The images present bacterial GFP fluorescence in green. SaMR12 colonize and aggregated on the surface of roots and root junction sites, followed by further transportation to aboveground tissues. Bars = 50 μm
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