Mutational impact of the CSI program on secondary metabolite gene clusters of P. chrysogenum. a Mutations acquired by Wisconsin 54-1255 in period 1 of the CSI programme are colored in red. The mutagenic impact in period 2 is shown in green. Mutated PKS and NRPS domains are indicated. The grey arrows represent other genes of the putative gene clusters. Abbreviations used for PKS and NRPS domains: KS, keto-synthase; AT, acyltransferase; DH, dehydratase; MT, methyltransferase; ER, enoyl reductase; KR, ketoreductase; ACP, acyl carrier protein; TE, thioesterase; TE/Red, thioester reductase; A, adenylation; T, thiolation; C, condensation domain. b Schematic representation of the Velvet complex proteins VelA, VelB and LaeA with the mutations obtained during the CSI
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