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Showing content from https://link.springer.com/article/10.1186/1471-2180-9-104/figures/2 below:

Figure 2 | Molecular characterization of a fungal gene paralogue of the penicillin penDE gene of Penicillium chrysogenum

Generation of the ial null mutant in P. chrysogenum. The transcription of the ial gene was blocked by insertion (double crossover; dashed lines) of the amdS selection marker in opposite orientation between the ial gene promoter and the ial ORF. Restriction enzymes indicated: Ba, BamHI; Sb, SbfI; Pm, PmeI.

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