Aβ injection reduces hippocampal neuronal area (Nissl staining) of 12-month-old wild-type (WT) mice but not tau knockout (KO) mice. (a) Representative images of the hippocampus (Hippo), CA1 region, and dentate gyrus (DG) region from treated mice. Only images of CA1 region and DG region were used for quantification. Scale bars: 250 μm for 5× and 50 μm for 40×. (b, c) Quantification of neuronal area in (b) CA1 and (c) DG regions. Detailed methods for quantification were described in the “Methods”. n (WT, phosphate-buffered saline) = 9; n (WT, Aβ) = 10; n (tau knockout, phosphate-buffered saline) = 5; n (tau knockout, Aβ) = 8. **p < 0.01 versus sham-injected WT mice; # p < 0.05 versus Aβ-injected WT mice. Data are means ± SEM
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