In this paper, a novel cell stretcher design that mimics the real-time stretch of the heart wall is introduced. By culturing cells under stretched conditions that mimics the mechanical aspects of the native cardiac environment, better understanding on the role of biomechanical signaling on cell development can be achieved. The device utilizes a moving magnet linear actuator controlled through pulse-width modulated power combined with an automated closed loop feedback system for accurate generation of a designated mechanical stretch profile. The system’s capability to stretch a cell culture membrane and accuracy of the designated frequency and waveform production for cyclic stretching were evaluated. Temperature and degradation assessments as well as a scalable design demonstrated the system’s cell culture application for long term, in vitro studies.
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Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. ReferencesBanes, A. J., J. Gilbert, D. Taylor, and O. Monbureau. A new vacuum-operated stress-providing instrument that applies static or variable duration cyclic tension or compression to cells in vitro. J. Cell Sci. 75:35–42, 1985.
Bernardo, B. C., K. L. Weeks, L. Pretorius, and J. R. McMullen. Molecular distinction between physiological and pathological hypertrophy cardiac hypertrophy: experimental findings and therapeutic strategies. Pharmacol. Ther. 128(1):191–227, 2010.
Brown, T. D. Techniques for mechanical stimulation of cells in vitro: a review. J. Biomech. 33:3–14, 2000.
Clark, R. E., D. S. Smith, P. H. Mellor, and D. Howe. Design optimization of moving-magnet actuators for reciprocating electro-mechanical systems. IEEE Trans. Magn. 31(6):3746–3748, 1995.
Colombo, A., P. A. Cahill, and C. Lally. An analysis of the strain field in biaxial Flexcell® membranes for different waveforms and frequencies. Proc. Inst. Mech. Eng. H 222(8):1235–1245, 2008.
Dorn, II, G. W. The fuzzy logic of physiological cardiac hypertrophy. Hypertension 49:962–970, 2007.
Ellis, E. F., J. S. McKinney, K. A. Willoughby, S. Kiang, and J. T. Povlishock. A new model for rapid stretch-induced injury of cells in culture: characterization of the model using astrocytes. J. Neurotrauma 12(3):325–339, 1995.
Lee, A. A., T. Delhaas, L. K. Waldman, D. A. MacKenna, F. J. Villarreal, and A. D. McCulloch. An equibiaxial strain system for cultured cells. Am. J. Physiol. Cell Physiol. 271(4):C1400–C1408, 1996.
Leung, D. Y. M., S. Glagov, and M. B. Matthews. A new in vitro system for studying cell response to mechanical stimulation. Exp. Cell Res. 109(2):285–298, 1977.
Sculz, R. M., and A. Bader. A cartilage tissue engineering and bioreactor systems for the cultivation and stimulation of chondrocytes. Eur. Biophys. J. 35:539–568, 2007.
Smith, K., S. A. Metzler, and J. N. Warnock. Cyclic strain inhibits acute pro-inflammatory gene expression in aortic valve interstitial cells. Biomech. Model. Mechanobiol. 9:117–125, 2010.
Sun, L., X. Wang, and D. L. Kaplan. A 3D cartilage—inflammatory cell culture system for the modeling of human osteoarthritis. Biomaterials 32(24):5581–5589, 2011.
Syedain, Z. H., J. S. Weinberg, and R. T. Tranquillo. Cyclic distension of fibrin-based tissue constructs: evidence of adaptation during growth of engineered connective tissue. Proc. Natl Acad. Sci. USA. 105(18):6537–6542, 2008.
Terracio, L., K. Rubin, D. Gullberg, E. Balog, W. Carver, R. Jyring, and T. K. Borg. Expression on collagen binding integrins during cardiac development and hypertrophy. Circ. Res. 68(3):734–744, 1991.
Terracio, L., A. Tingstrom, W. H. Peters, III, and T. K. Borg. A potential role for mechanical stimulation in cardiac development. Ann. N.Y. Acad. Sci. 588(1):48–60, 2006.
Thompson, M. T. Practical issues in the use of NdFeB permanent magnets in maglev, motors, bearings, and eddy current brakes. Proc. IEEE 97(11):1758–1767, 2009. doi:10.1109/JPROC.2009.2030231.
Vandenburgh, H. H., and P. Karlisch. Longitudinal growth of skeletal myotubes in vitro in a new horizontal mechanical stimulator. In Vitro Cell. Dev. Biol. 25(7):607–616, 1989.
Vogel, M., D. B. McElhinney, E. Marcus, D. Morash, R. W. Jennings, and W. Tworetzky. Significance and outcome of left heart hypoplasia in fetal congenital diaphragmatic hernia. Ultrasound Obstet. Gynecol. 35(3):310–317, 2010.
Ye, K. Y., K. E. Sullivan, and L. D. Black III. Encapsulation of cardiomyocytes in a fibrin hydrogel for cardiac tissue engineering. J. Vis. Exp. 55(e3251):1–7, 2011.
This work was funded by grants from the American Heart Association (Summer Fellowship to RMW) and the NIH-NHLBI (Awards R21HL115570 and R00HL093358 to LDB).
Author information Authors and AffiliationsDepartment of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA, 02155, USA
Jason J. Lau, Raymond M. Wang & Lauren D. Black III
Cell, Developmental and Molecular Biology Program, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA, 02215, USA
Lauren D. Black III
Correspondence to Lauren D. Black III.
Additional informationAssociate Editor Umberto Morbiducci oversaw the review of this article.
Jason J. Lau and Raymond M. Wang contributed equally to this work.
About this article Cite this articleLau, J.J., Wang, R.M. & Black, L.D. Development of an Arbitrary Waveform Membrane Stretcher for Dynamic Cell Culture. Ann Biomed Eng 42, 1062–1073 (2014). https://doi.org/10.1007/s10439-014-0976-x
Received: 31 October 2013
Accepted: 10 January 2014
Published: 29 January 2014
Issue Date: May 2014
DOI: https://doi.org/10.1007/s10439-014-0976-x
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