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The Use of SU-8 Topographically Guided Microelectrode Array in Measuring Extracellular Field Potential Propagation

Abstract

The microelectrode array (MEA) can be used to study extracellular field potentials (exFPs) of electrogenic cells. Microcontact printing, which must be repeated after each experiment, is often used to promote accurate positioning of cells onto electrodes. The present study used MEAs with evenly spaced detection electrodes aligning along permanent SU-8 topographical guidance channels to measure propagation direction and speed. Chronotropic agents, isoproterenol (ISO, 1 nM–1 mM), and verapamil (VP, 1 nM–10 μM); and potassium channel openers (KCOs), pinacidil (PIN), and SDZ PCO400 (SDZ), were used to characterize these MEA chips. ISO (1 mM) enhanced the propagation speed from 247.25 ± 50.58 μm/ms 381.29 ± 92.01 μm/ms (n = 9, p < 0.05), whereas VP (10 μM) reduced the propagation speed completely (n = 12, p < 0.001). PIN (1 mM) significantly reduced the propagation speed from 278.6 ± 43.7 μm/ms to 49.7 ± 27.7 μm/ms (n = 10, p < 0.001), whereas SDZ (1 mM) completely stopped the propagation (n = 9, p < 0.001). Both KCOs induced conduction pattern changes similar to those observed in cardiac arrhythmia. The MEA chips with SU-8 guidance channels may be used to study cardiovascular diseases that are related to conduction disruption.

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Acknowledgments

This study is supported by the Earmarked Grant from the Research Grant Council of Hong Kong under the contract number 611205 (awarded to M. Chan, HKUST) and the Helmholtz Association of National Research Centres, Germany. The authors would like to thank N. Wolters (IBN-2, Electronic Workshop) and Y. Zhang (IBN-2) for their technical expertise in designing the current MEA system.

Author information Authors and Affiliations

  1. Bioengineering Graduate Program, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Hong Kong

    Jessica Ka-Yan Law & Mansun Chan

  2. School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong (CUHK), Room 405L, Basic Medical Sciences Building, Shatin, Hong Kong

    Chi-Kong Yeung & John Anthony Rudd

  3. Department of Electronic and Computer Engineering, The Hong Kong University of Science and Technology (HKUST), Clear Water Bay, Hong Kong

    Lin Li & Mansun Chan

  4. Department of Informatics and Microsystem Technology, University of Applied Sciences Kaiserslautern, D-66482, Zweibrücken, Germany

    Sven Ingebrandt

Authors
  1. Jessica Ka-Yan Law
  2. Chi-Kong Yeung
  3. Lin Li
  4. John Anthony Rudd
  5. Sven Ingebrandt
  6. Mansun Chan
Corresponding author

Correspondence to Chi-Kong Yeung.

Additional information

Associate Editor Bahman Anvari oversaw the review of this article.

About this article Cite this article

Law, J.KY., Yeung, CK., Li, L. et al. The Use of SU-8 Topographically Guided Microelectrode Array in Measuring Extracellular Field Potential Propagation. Ann Biomed Eng 40, 619–627 (2012). https://doi.org/10.1007/s10439-011-0432-0

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