This study evaluated the possible clinical application of low-intensity ultrasound (LIUS) stimulation for preventing osteoporotic bone fracture. Eight virgin 14-week-old ICR mice (weight 24.0 ± 0.7 g) were ovariectomized to induce osteoporosis. The right hind limbs (US limbs) were stimulated with LIUS, whereas the left hind limbs (CON limbs) were not stimulated. LIUS was applied for 20 min a day, 5 days a week over a 6-week period using the following parameters: 1.5 MHz frequency, 1.0 kHz pulse repetition, 30 mW/cm2 intensity, and 200 μs pulse length. The effective structural modulus increased significantly (p < 0.05) in the US limbs over time with the increased bone quantity, whereas that in CON limbs remained statistically constant (p > 0.05). In addition, the elastic modulus in the US limbs was generally enhanced by an increased bone quality, compared with the CON limbs. Therefore, LIUS stimulation may effectively reduce the risk of osteoporotic bone fracture by increasing the mechanical characteristics of bone via improvements in both the effective structural and elastic modulus of the osteoporotic bone. In conclusion, LIUS may potentially prove very effective clinically for preventing osteoporotic bone fractures.
This is a preview of subscription content, log in via an institution to check access.
Access this article Subscribe and saveSpringer+ Basic
€34.99 /Month
Price includes VAT (Germany)
Instant access to the full article PDF.
Similar content being viewed by others Explore related subjectsDiscover the latest articles and news from researchers in related subjects, suggested using machine learning. ReferencesAynaci, O., et al. The effect of ultrasound on the healing of muscle-pediculated bone graft in spinal fusion. Spine 27:1531–1535, 2002.
Azuma, Y., et al. Low-intensity pulsed ultrasound accelerates rat femoral fracture healing by acting on the various cellular reactions in the fracture callus. J. Bone Miner. Res. 16:671–680, 2001.
Briggs, A. M., et al. The effect of osteoporotic vertebral fracture on predicted spinal loads in vivo. Eur. Spine J. 15:1785–1795, 2006.
Carvalho, D. C., and A. Cliquet Jr. The action of low-intensity pulsed ultrasound in bones of osteopenic rats. Artif. Org. 28:114–118, 2004.
Elliott, D. M., and J. J. Saver. Young investigator award winner: validation of the mouse and rat disc as mechanical models of the human lumbar disc. Spine 29:713–722, 2004.
Gerasimenko, Y., et al. Epidural stimulation: comparison of the spinal circuits that generate and control locomotion in rats, cats and humans. Exp. Neurol. 209(2):417–425, 2008.
Hantes, M. E., et al. Low-intensity transosseous ultrasound accelerates osteotomy healing in a sheep fracture model. J. Bone Joint Surg. 86:2275–2282, 2004.
Heckman, J. D., et al. Acceleration of tibial fracture-healing by non-invasive, low-intensity pulsed ultrasound. J. Bone Joint Surg. 76:26–34, 1994.
Kaufman, J. J., et al. Ultrasound simulation in bone. IEEE Trans. Ultrason. Ferroelectr. Freq. Control 55:1205–1218, 2008.
Kinney, J. H., et al. Three-dimensional morphometry of the L6 vertebra in the ovariectomized rat model of osteoporosis: biomechanical implications. J. Bone Miner. Res. 15:1981–1991, 2000.
Kristiansen, T. K., et al. Accelerated healing of distal radial fractures with the use of specific, low-intensity ultrasound. A multicenter, prospective, randomized, double-blind, placebo-controlled study. J. Bone Joint Surg. 79:961–973, 1997.
Laib, A., et al. 3D micro-computed tomography of trabecular and cortical bone architecture with application to a rat model of immobilisation osteoporosis. Med. Biol. Eng. Comput. 38:326–332, 2000.
Mosekilde, L. Consequences of the remodelling process for vertebral trabecular bone structure: a scanning electron microscopy study (uncoupling of unloaded structures). Bone Miner. 10:13–35, 1990.
Neer, R. M., et al. Effect of parathyroid hormone (1–34) on fractures and bone mineral density in postmenopausal women with osteoporosis. N. Engl. J. Med. 344:1434–1441, 2001.
Phillips, F. M., et al. In vivo BMP-7 (OP-1) enhancement of osteoporotic vertebral bodies in an ovine model. Spine J. 6:500–506, 2006.
Pilla, A. A., et al. Non-invasive low-intensity pulsed ultrasound accelerates bone healing in the rabbit. J. Orthop. Trauma 4:246–253, 1990.
Rho, J. Y., et al. Relations of mechanical properties to density and CT numbers in human bone. Med. Eng. Phys. 17:347–355, 1995.
Rubin, C. T., and L. E. Lanyon. Regulation of bone formation by applied dynamic loads. J. Bone Joint Surg. 66-A:397–402, 1984.
Rutten, S., et al. Low-intensity pulsed ultrasound increases bone volume, osteoid thickness and mineral apposition rate in the area of fracture healing in patients with a delayed union of the osteotomized fibula. Bone 43:348–354, 2008.
Sample, S. J., et al. Functional adaptation to loading of a single bone is neuronally regulated and involves multiple bones. J. Bone Miner. Res. 23:1372–1381, 2008.
Shiraishi, A., et al. A comparison of alfacalcidol and menatetrenone for the treatment of bone loss in an ovariectomized rat model of osteoporosis. Calcif. Tissue Int. 71(1):69–79, 2002.
Siffert, R. S., and J. J. Kaufman. Ultrasonic bone assessment: “the time has come”. Bone 40:5–8, 2007.
Turner, C. H. Three rules for bone adaptation to mechanical stimuli. Bone 23:399–407, 1998.
Ulrich, D., et al. Finite element analysis of trabecular bone structure: a comparison of image-based meshing techniques. J. Biomech. 31:1187–1192, 1998.
Umemura, Y., et al. Five jumps per day increase bone mass and breaking force in rats. J. Bone Miner. Res. 12:1480–1485, 1997.
Wang, S. J., et al. Low intensity ultrasound treatment increases strength in a rat femoral fracture model. J. Orthop. Res. 12:40–47, 1994.
Warden, S. J., et al. Skeletal effects of low-intensity pulsed ultrasound on the ovariectomized rodent. Ultrasound Med. Biol. 27:989–998, 2001.
Wimsatt, J., et al. Ultrasound therapy for the prevention and correction of contractures and bone mineral loss associated with wing bandaging in the domestic pigeon (Columba livia). J. Zoo Wildlife Med. 31:190–195, 2000.
Woo, D. G., et al. Relationship between nutrition factors and osteopenia: Effects of experimental diets on immature bone quality. J. Biomech. 42:1102–1107, 2009.
Yang, K. H., and S. J. Park. Stimulation of fracture healing in a canine ulna full-defect model by low-intensity pulsed ultrasound. Yonsei Med. J. 42:503–508, 2001.
This study was supported by a grant of the Korea Healthcare technology R&D Project, Ministry for Health, Welfare &Family Affairs, Republic of Korea (A080920).
Author information Authors and AffiliationsDepartment of Biomedical Engineering, Yonsei University, Wonju, Gangwon, 220-710, Republic of Korea
Dae Gon Woo, Chang-Yong Ko, Han Sung Kim & Jong Bum Seo
Gerontechnology Center, Korea Institute of Industrial Technology, 35-3, Hongcheon, Ipjang, Cheonan, Chungnam, 330-825, Republic of Korea
Dohyung Lim
Correspondence to Dohyung Lim.
Additional informationAssociate Editor Eiji Tanaka oversaw the review of this article.
Dae Gon Woo and Chang-Yong Ko contributed to this work as First Author.
About this article Cite this articleWoo, D.G., Ko, CY., Kim, H.S. et al. Evaluation of the Potential Clinical Application of Low-Intensity Ultrasound Stimulation for Preventing Osteoporotic Bone Fracture. Ann Biomed Eng 38, 2438–2446 (2010). https://doi.org/10.1007/s10439-010-9983-8
Received: 20 October 2009
Accepted: 22 February 2010
Published: 05 March 2010
Issue Date: July 2010
DOI: https://doi.org/10.1007/s10439-010-9983-8
RetroSearch is an open source project built by @garambo | Open a GitHub Issue
Search and Browse the WWW like it's 1997 | Search results from DuckDuckGo
HTML:
3.2
| Encoding:
UTF-8
| Version:
0.7.4