Pc12g03090 is a novel fumarate reductase-cytochrome b5 fusion protein with a putative internal PTS2 sequence that locates to microbodies in P. chrysogenum. a Schematic representation of the structure of P. chrysogenum Pc12g03090. This protein consists of a fumarate reductase domain, a cytochrome b5 heme binding domain, as well as a linker region containing a putative PTS2. This internal PTS2 is conserved in many organisms, mainly filamentous fungi. An alignment of representative PTS2 sequences is also shown. These sequences were taken from the alignment in Supplementary Fig. S2. Conserved residues are shaded. b Localization of Pc12g03090 in P. chrysogenum. P. chrysogenum DsRed.SKL eGFP.Pc12g03090 cells were cultivated on penicillin production medium supplemented with phenoxyacetic acid for 2 days and analyzed by confocal laser scanning fluorescence microscopy. The GFP fluorescence completely co-localized with the DsRed fluorescence, indicating that the fusion protein is present in microbodies. For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article
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