P. chrysogenum orthologs of CefD1 (Pc20g13500) and CefD2 (Pc22g13680) localize to microbodies. P. chrysogenum DsRed.SKL eGFP.Pc20g13500 (a) and DsRed.SKL eGFP.Pc22g13680 (b) cells were cultivated on penicillin production medium supplemented with phenoxyacetic acid for 30 h and analyzed by confocal laser scanning fluorescence microscopy. In both cases, GFP and DsRed fluorescence co-localized, indicating that both fusion proteins are present in microbodies. DsRed.SKL eGFP.Pc22g13680 cells also showed a faint cytosolic eGFP fluorescence, indicating that a portion of the eGFP.Pc22g13680 fusion protein is located in the cytosol. For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article
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