When I performed motif footprinting analysis from the signac footprinting vignette in my own dataset, the motif object set in seurat was 0x0 and there occur an error in functon PlotFootprint: Error in (ncol(x = x) - background):ncol(x = x) : argument of length 0.
So I created motif assay directly in Motif analysis vignette , and then run footprint and PlotFootprint function, but it still occur an error: Error in (ncol(x = x) - background):ncol(x = x) : argument of length 0.
code:
tmp <- Footprint(
object = tmp ,
motif.name = features,
genome = BSgenome.Hsapiens.UCSC.hg38
)
PlotFootprint(tmp, features = features)
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