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Showing content from https://github.com/stuart-lab/signac/issues/1509 below:

Possbile Signac::FilterCells bug · Issue #1509 · stuart-lab/signac · GitHub

hey,

thanks a lot for developing and maintaining this very helpful package!!!!

i am possibly observing a bug in the Signac::FilterCells function.

given a test fragments file

zcat fragments.tsv.gz
chr1	9989	10162	GCTGCAATCCAGGGAG-1	1
chr10	25101623	25101727	GCTGCAATCCAGGGAG-1	1

and running the code

 Signac::FilterCells("fragments.tsv.gz",c("GCTGCAATCCAGGGAG-1","GCTGCAATCCAGGGAG-1"),"test.gz",verbose=T)

Keeping 1 cell barcodes

Compressing filtered file
Indexing fragment file

i am obtaining a unexpected filtered fragment file, with a duplication of one of the entries

zcat test.gz
chr1	9989	10162	GCTGCAATCCAGGGAG-1	1
chr1	9989	10162	GCTGCAATCCAGGGAG-1	1
chr10	25101623	25101727	GCTGCAATCCAGGGAG-1	1

it might all come from here:
https://github.com/stuart-lab/signac/blob/master/src/filter.cpp

R version 4.2.2 (2022-10-31)
Signac_1.9.0

thanks for your help!


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