Hi,
I am trying to create the gene activity matrix for not only protein-coding regions but also other mentioned biotypes such as biotypes = c("protein_coding", "lincRNA", "rRNA", "processed_transcript"). But whenever i try to change the biotypes, i get following error:
> gene.activities <- GeneActivity(
+ pbmc,
+ assay = NULL,
+ features = NULL,
+ extend.upstream = 2000,
+ extend.downstream = 0,
+ biotypes = c("protein_coding", "lincRNA", "rRNA", "processed_transcript"),
+ max.width = 5e+05,
+ gene.id = FALSE,
+ verbose = TRUE
+ )
Extracting gene coordinates
Error in FeatureMatrix(fragments = Fragments(object = object[[assay]]), :
unused arguments (biotypes = c("protein_coding", "lincRNA", "rRNA", "processed_transcript"), max.width = 5e+05, gene.id = FALSE)
It would be great if you could suggest to me how to include other biotypes.
Thank you
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