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Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation

Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation

Nature volume 362, pages 175–179 (1993)Cite this article

Abstract

BASAL transcription by human RNA polymerase II requires the coordinate action of several ancillary factors (TFIIA-J)1 and can be regulated by various promoter-specific DNA binding proteins. An additional class of factors, called coactivators, are dispensable for basal transcription but are indispensable for regulation by transcriptional activators2–4. Biochemical studies established that some coactivators are associated with the TATA-binding protein (TBP) to form the TFIID complex3–6. We therefore set out to define the relationship between TBP and these TBP-associated factors (TAFs). Here we describe the cloning, expression and properties of the first human TAF, hTAFII250. The hTAFII250 gene is identical to a gene, CCG1 (refs 7, 8), implicated in cell-cycle progression. Recombinant hTAFII250 binds directly to TBP both in vitro and in yeast, and participates in the formation of the TFIID complex. This largest TAF may therefore play a central role in TFIID assembly by interacting with both TBP and other TAFs, as well as serving to link the control of transcription to the cell cycle.

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Author information Authors and Affiliations

  1. Howard Hughes Medical Institute, Department of Molecular and Cell Biology, 401 Barker Hall, University of California at Berkeley, Berkeley, California, 94720, USA

    Siegfried Ruppert, Edith H. Wang & Robert Tjian

Authors
  1. Siegfried Ruppert
  2. Edith H. Wang
  3. Robert Tjian
About this article Cite this article

Ruppert, S., Wang, E. & Tjian, R. Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation. Nature 362, 175–179 (1993). https://doi.org/10.1038/362175a0

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