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README

The tidyHeatmap package

Stefano Mangiola 2025-01-26

tidyHeatmap is a package that introduces tidy principles to the creation of information-rich heatmaps. This package uses ComplexHeatmap as graphical engine.

Citation

Mangiola et al., (2020). tidyHeatmap: an R package for modular heatmap production based on tidy principles. Journal of Open Source Software, 5(52), 2472, https://doi.org/10.21105/joss.02472

Please have a look also to

tidygate for adding custom gate information to your tibble
tidySingleCellExperiment for tidy manipulation of Seurat objects
tidyseurat for tidy manipulation of Seurat objects
tidybulk for tidy high-level data analysis and manipulation
tidySummarizedExperiment for heatmaps produced with tidy principles

Full documentation here

# Create some more data points
pasilla_plus <- 
    tidyHeatmap::pasilla |>
    dplyr::mutate(activation_2 = activation, activation_3 = activation) |> 
    tidyr::nest(data = -sample) |>
    dplyr::mutate(size = rnorm(n(), 4,0.5)) |>
    dplyr::mutate(age = runif(n(), 50, 200)) |>
    tidyr::unnest(data) 

# Plot
pasilla_plus |>
    heatmap(
        .column = sample,
        .row = symbol,
        .value = `count normalised adjusted`,   
        scale = "row"
    ) |>
    annotation_tile(condition) |>
    annotation_point(activation) |>
    annotation_numeric(activation_3) |>
    annotation_tile(activation_2) |>
    annotation_bar(size) |>
    annotation_line(age)

Advantages:

Modular annotation with just specifying column names
Custom grouping of rows is easy to specify providing a grouped tbl. For example df |> group_by(...)
Labels size adjusted by row and column total number
Default use of Brewer and Viridis palettes

Functions/utilities available heatmap Plots base heatmap group_by dplyr function - groups heatpmap rows/columns annotation_tile Adds tile annotation to the heatmap annotation_point Adds point annotation to the heatmap annotation_bar Adds bar annotation to the heatmap annotation_numeric Adds bar + number annotation to the heatmap annotation_line Adds line annotation to the heatmap layer_text Add layer of text on top of the heatmap layer_point Adds layer of symbols on top of the heatmap layer_square Adds layer of symbols on top of the heatmap layer_diamond Adds layer of symbols on top of the heatmap layer_arrow_up Adds layer of symbols on top of the heatmap layer_arrow_down Add layer of symbols on top of the heatmap layer_star Add layer of symbols on top of the heatmap layer_asterisk Add layer of symbols on top of the heatmap split_rows Splits the rows based on the dendogram split_columns Splits the columns based on the dendogram save_pdf Saves the PDF of the heatmap + Integrate heatmaps side-by-side as_ComplexHeatmap Convert the tidyHeatmap output to ComplexHeatmap for non-standard âdrawingâ wrap_heatmap Allows the integration with the patchwork package Installation

To install the most up-to-date version

devtools::install_github("stemangiola/tidyHeatmap")

To install the most stable version (however please keep in mind that this package is under a maturing lifecycle stage)

install.packages("tidyHeatmap")

Contribution

If you want to contribute to the software, report issues or problems with the software or seek support please open an issue here

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