This is the development version of scifer; for the stable release version, see scifer.
Scifer: Single-Cell Immunoglobulin Filtering of Sanger SequencesBioconductor version: Development (3.22)
Have you ever index sorted cells in a 96 or 384-well plate and then sequenced using Sanger sequencing? If so, you probably had some struggles to either check the electropherogram of each cell sequenced manually, or when you tried to identify which cell was sorted where after sequencing the plate. Scifer was developed to solve this issue by performing basic quality control of Sanger sequences and merging flow cytometry data from probed single-cell sorted B cells with sequencing data. scifer can export summary tables, 'fasta' files, electropherograms for visual inspection, and generate reports.
Author: Rodrigo Arcoverde Cerveira [aut, cre, cph] ORCID: 0000-0002-1145-2534 , Marcel Martin [ctb], Matthew James Hinchcliff [ctb], Sebastian Ols [aut, dtc] ORCID: 0000-0001-9784-7176 , Karin Loré [dtc, ths, fnd] ORCID: 0000-0001-7679-9494
Maintainer: Rodrigo Arcoverde Cerveira <rodrigo.arcoverdi at gmail.com>
Citation (from within R, entercitation("scifer")): Installation
To install this package, start R (version "4.5") and enter:
if (!require("BiocManager", quietly = TRUE))
install.packages("BiocManager")
# The following initializes usage of Bioc devel
BiocManager::install(version='devel')
BiocManager::install("scifer")
For older versions of R, please refer to the appropriate Bioconductor release.
DocumentationTo view documentation for the version of this package installed in your system, start R and enter:
browseVignettes("scifer")Follow Installation instructions to use this package in your R session.
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