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Showing content from http://www.ncbi.nlm.nih.gov/IEB/ToolBox/CPP_DOC/doxyhtml/blastxml__format_8cpp_source.html below:

NCBI C++ ToolKit: src/algo/blast/format/blastxml_format.cpp Source File

59

ncbi::TMaskedQueryRegions

mask

;

74  bool

add_reference_dtdi =

false

,

75  bool

add_xml_versioni =

false

);

91

frame = (start - 1) % 3 + 1;

93

frame = -((seq_length - end) % 3 + 1);

111  const

ncbi::TMaskedQueryRegions* mask_info,

112  int

master_gentic_code,

int

slave_genetic_code)

120  bool

query_is_na, subject_is_na;

121  int

query_length, subject_length;

123  int

score, num_ident;

127

list<TGi> use_this_gi;

128

CBlastFormatUtil::GetAlnScores(kAlign, score, bit_score, evalue, sum_n,

129

num_ident, use_this_gi);

133

sprintf(

tmp

,

"%.*g"

, 6, bit_score );

134

bit_score = atof(

tmp

);

135

sprintf(

tmp

,

"%.*g"

, 6, evalue );

136

evalue = atof(

tmp

);

138

xhsp->SetBit_score(bit_score);

139

xhsp->SetScore(score);

140

xhsp->SetEvalue(evalue);

146

query_is_na = kQueryBioseqHandle.

IsNa

();

150

subject_is_na = kSubjBioseqHandle.

IsNa

();

156

xhsp->SetQuery_from(0);

157

xhsp->SetQuery_to(0);

158

xhsp->SetHit_from(0);

160

xhsp->SetIdentity(num_ident);

164

xhsp_list.push_back(xhsp);

178  if

(query_is_na && subject_is_na)

181

final_aln = densegAln;

199  string

masked_query_seq;

206  const bool

kIsBlastn =

207

(query_is_na && subject_is_na && !kTranslated);

218

reversed_ds->

Assign

(kDenseg);

220

ds_pt = &(*reversed_ds);

223  int

q_start, q_end, s_start, s_end, q_frame=0, s_frame=0;

225  unsigned int

num_gaps = 0;

226  int

align_length = 0;

249  if

(!kTranslated && query_is_na && subject_is_na) {

250

q_frame = s_frame = 1;

259

}

else if

(kTranslated) {

265

q_start, q_end, query_length);

268

s_start, s_end, subject_length);

271

xhsp->SetQuery_frame(q_frame);

272

xhsp->SetHit_frame(s_frame);

274

xhsp->SetQuery_from(q_start);

275

xhsp->SetQuery_to(q_end);

276

xhsp->SetHit_from(s_start);

277

xhsp->SetHit_to(s_end);

306  int

num_positives = 0;

307

middle_seq = query_seq;

313  const unsigned int

kMaxOffset =

static_cast<unsigned int>

(

min

(query_seq.size(),

314

subject_seq.size()));

315  for

(

unsigned int i

= 0;

i

< kMaxOffset; ++

i

) {

316  if

(query_seq[

i

] == subject_seq[

i

]) {

320

middle_seq[

i

] =

'|'

;

322

(*matrix)(query_seq[

i

], subject_seq[

i

]) > 0 &&

325

middle_seq[

i

] = kIsBlastn ?

' '

:

'+'

;

327

middle_seq[

i

] =

' '

;

331

xhsp->SetIdentity(num_ident);

332

xhsp->SetGaps(num_gaps);

333

xhsp->SetAlign_len(align_length);

336

xhsp->SetQseq(masked_query_seq);

338

xhsp->SetQseq(query_seq);

339

xhsp->SetHseq(subject_seq);

340

xhsp->SetMidline(middle_seq);

341

xhsp->SetPositive(num_positives);

344

xhsp_list.push_back(xhsp);

359  const

ncbi::TMaskedQueryRegions* mask_info,

360  bool

ungapped,

int

master_gentice_code,

int

slave_genetic_code)

366  if

(kAlignSet.

Get

().empty())

372  const CSeq_id

& kSeqId = kAlignSet.

Get

().front()->GetSeq_id(1);

378 

list<TGi> use_this_gi;

387

defline =

"No definition line"

;

390

hit->SetDef(defline);

396

hit->SetAccession(accession);

405

hit->SetDef(

"Unknown"

);

406

hit->SetAccession(

"Unknown"

);

420

matrix, mask_info, master_gentice_code, slave_genetic_code);

423

mask_info, master_gentice_code, slave_genetic_code);

455  const

ncbi::TMaskedQueryRegions* mask_info,

456  bool

ungapped,

int

master_gentice_code,

int

slave_genetic_code,

460  if

(alnset.

Get

().empty())

463

CSeq_align_set::Tdata::const_iterator iter = alnset.

Get

().begin();

466  bool

incremental_output = (

bool

)out_stream;

467  while

(iter != alnset.

Get

().end()) {

474  if

((*iter)->GetSegs().IsDisc()) {

476

ungapped, master_gentice_code, slave_genetic_code);

481  for

( ; iter != alnset.

Get

().end(); ++iter) {

483  if

(!current_id->

Match

(*next_id)) {

486

one_subject_alnset.

Set

().push_back(*iter);

489

disc_align_wrap.

SetSegs

().SetDisc(one_subject_alnset);

491

mask_info, ungapped, master_gentice_code, slave_genetic_code);

495

new_hit->SetNum(index);

497  if

( !incremental_output ) hits.push_back(new_hit);

503

xml_one_hit_os->SetReferenceDTD(

false

);

504

xml_one_hit_os->Write( &(*new_hit), new_hit->GetThisTypeInfo() );

507

string::size_type start_xml_pos = out_str.find(

"<?xml"

);

508  if

( start_xml_pos != string::npos ) {

509

string::size_type end_xml_pos = out_str.find_first_of(

"\n\r"

);

510

out_str.erase(0,end_xml_pos+1);

512

*out_stream << out_str ;

535  const

ncbi::TMaskedQueryRegions* mask_info,

536  int

index,

int

iteration,

CStatistics

& stat,

bool

is_ungapped,

537  int

master_gentice_code,

int

slave_genetic_code,

538  const

vector<string>& messages,

541  bool

incremental_output = (

bool

) out_stream;

542

list<CRef<CIteration> >& iterations = bxmlout.

SetIterations

();

546

one_query_iter->SetIter_num(iteration);

557

one_query_iter->SetQuery_ID(

559

query_def = sequence::CDeflineGenerator().GenerateDefline(bh);

565

query_def =

"No definition line"

;

566

one_query_iter->SetQuery_def(query_def);

569

one_query_iter->SetStat(stat);

570  if

(messages.size() > 0 && !messages[index].empty())

571

one_query_iter->SetMessage(messages[index]);

573  string

serial_xml_start, serial_xml_end;

574  if

( incremental_output) {

577

serial_xml_start, serial_xml_end);

578

*out_stream << serial_xml_start <<

"\n<Iteration_hits>\n"

;

584

scope, matrix, mask_info, is_ungapped,

585

master_gentice_code, slave_genetic_code,

589  if

( incremental_output ) *out_stream <<

"</Iteration_hits>"

<< serial_xml_end;

591

iterations.push_back(one_query_iter);

602  string

matrix_name =

data

->GetMatrixName();

610  if

((

val

=

data

->GetMatchReward()) != 0)

613  if

((

val

=

data

->GetMismatchPenalty()) != 0)

632  int

db_numseq =

data

->GetDbNumSeqs();

633  Int8

db_length =

data

->GetDbLength();

635  for

(

unsigned int

index = 0; index <

data

->GetNumQueries(); ++index) {

644

stat_vec.push_back(stat);

681  bool

incremental_output = (

bool

)out_stream;

682  string

program_name =

data

->GetBlastProgramName();

687

bxmlout.

SetDb

(

data

->GetDatabaseName());

705

query_def = sequence::CDeflineGenerator().GenerateDefline(bh);

711

query_def =

"No definition line"

;

719

unique_ptr< CBlastFormattingMatrix > matrix(

data

->GetMatrix());

721

vector<CRef<CStatistics> > stat_vec;

724  string

serial_xml_start, serial_xml_end;

725  if

( incremental_output && incremental_struct->

m_IterationNum

== 0) {

726  bool

add_dtd_reference =

true

, add_xml_version =

true

;

728

serial_xml_start, serial_xml_end,

729

add_dtd_reference, add_xml_version );

731

*out_stream << serial_xml_start <<

"\n<BlastOutput_iterations>"

;

732

incremental_struct->

m_SerialXmlEnd

=

"\n</BlastOutput_iterations>"

+ serial_xml_end;

735  for

(

unsigned int

index = 0; index <

data

->GetNumQueries(); ++index) {

743  if

(incremental_struct)

749  data

->GetScope(index), matrix.get(),

750  data

->GetMaskLocations(index),

752

*stat_vec[index], !

data

->GetGappedMode(),

753  data

->GetMasterGeneticCode(),

data

->GetSlaveGeneticCode(),

754  data

->GetMessages(),

758  ERR_POST

(

Error

<<

"Failed s_BlastXMLAddIteration "

<< query_label << e.

what

() );

762  ERR_POST

(

Error

<<

"Failed s_BlastXMLAddIteration "

<< query_label );

775  bool

add_reference_dtd,

776  bool

add_xml_version )

778  bool

res_code =

false

;

779  TTypeInfo

typeInfo =

object

.GetThisTypeInfo();

780  string

breake_by_tag =

tag

;

781

start_part=

"<NOT SET>"

;

782

end_part=

"</NOT SET>"

;

788

xml_one_iter_os->SetReferenceDTD(add_reference_dtd);

789

xml_one_iter_os->SetDefaultDTDFilePrefix(

"http://www.ncbi.nlm.nih.gov/dtd/"

);

790  if

( add_xml_version )

791

xml_one_iter_os->Write(&

object

, typeInfo );

793

xml_one_iter_os->WriteObject(&

object

, typeInfo );

796

string::size_type iterations_insert_point = out_str.find( breake_by_tag );

797  if

( iterations_insert_point != string::npos ){

798

iterations_insert_point += breake_by_tag.length();

799

start_part = out_str.substr(0,iterations_insert_point);

800

end_part = out_str.substr(iterations_insert_point);

804

start_part = out_str;

User-defined methods of the data storage class.

Declares singleton objects to store the version and reference for the BLAST engine.

BLAST formatter utilities.

Formatting of pairwise sequence alignments in XML form.

Declares class to display one-line descriptions at the top of the BLAST report.

EProgram

This enumeration is to evolve into a task/program specific list that specifies sets of default parame...

@ ePHIBlastn

Nucleotide PHI BLAST.

@ ePHIBlastp

Protein PHI BLAST.

@ eMapper

Jumper alignment for mapping.

@ eMegablast

Nucl-Nucl (traditional megablast)

@ eDeltaBlast

Delta Blast.

static void s_SeqAlignSetToXMLHits(list< CRef< CHit > > &hits, const CSeq_align_set &alnset, CScope *scope, const CBlastFormattingMatrix *matrix, const ncbi::TMaskedQueryRegions *mask_info, bool ungapped, int master_gentice_code, int slave_genetic_code, CNcbiOstream *out_stream)

Fills the list of CHit objects, given a list of Seq-aligns.

static void s_BlastXMLGetStatistics(vector< CRef< CStatistics > > &stat_vec, const IBlastXMLReportData *data)

Fills the search statistics part of the BLAST XML output for all queries.

static void s_SetBlastXMLParameters(CBlastOutput &bxmlout, const IBlastXMLReportData *data)

Fills the parameters part of the BLAST XML output.

void BlastXML_FormatReport(CBlastOutput &bxmlout, const IBlastXMLReportData *data, CNcbiOstream *out_stream, SBlastXMLIncremental *incremental_struct)

Fills all fields in the data structure for a BLAST XML report.

static void s_SeqAlignToXMLHit(CRef< CHit > &hit, const CSeq_align &align_in, CScope *scope, const CBlastFormattingMatrix *matrix, const ncbi::TMaskedQueryRegions *mask_info, bool ungapped, int master_gentice_code, int slave_genetic_code)

Fill the CHit object in BLAST XML output, given an alignment and other information.

static int s_GetTranslationFrame(bool plus_strand, int start, int end, int seq_length)

Returns translation frame given the strand, alignment endpoints and total sequence length.

static CReference::EPublication s_GetBlastPublication(EProgram program)

Given BLAST task, returns enumerated value for the publication to be referenced.

static void s_SeqAlignSetToXMLHsps(list< CRef< CHsp > > &xhsp_list, const CSeq_align_set &alnset, CScope *scope, const CBlastFormattingMatrix *matrix, const ncbi::TMaskedQueryRegions *mask_info, int master_gentic_code, int slave_genetic_code)

Creates a list of CHsp structures for the XML output, given a list of Seq-aligns.

static const CSeq_id * s_GetSubjectId(const CSeq_align &align)

Retrieves subject Seq-id from a Seq-align.

ncbi::TMaskedQueryRegions mask

static bool s_SerializeAndSplitBy(const CSerialObject &object, const char *tag, string &start_part, string &end_part, bool add_reference_dtdi=false, bool add_xml_versioni=false)

serialize givem object and split data by provided XML tag for futher manual integrationa

static void s_BlastXMLAddIteration(CBlastOutput &bxmlout, const CSeq_align_set *alnset, const CSeq_loc &seqloc, CScope *scope, const CBlastFormattingMatrix *matrix, const ncbi::TMaskedQueryRegions *mask_info, int index, int iteration, CStatistics &stat, bool is_ungapped, int master_gentice_code, int slave_genetic_code, const vector< string > &messages, CNcbiOstream *out_stream)

Add an "iteration" to the BLAST XML report, corresponding to all alignments for a single query.

static string GetLabel(CConstRef< objects::CSeq_id > id, bool with_version=false)

Return a label for an ID Tries to recreate behavior of GetLabel before a change that prepends "ti|" t...

256x256 matrix used for calculating positives etc.

void Reverse(void)

Reverse the segments' orientation.

void Assign(const CSerialObject &obj, ESerialRecursionMode how=eRecursive)

overloaded Assign()

static CRef< objects::CSeq_align_set > PrepareBlastUngappedSeqalign(const objects::CSeq_align_set &alnset)

static functions Need to call this if the seqalign is stdseg or dendiag for ungapped blast alignment ...

SeqLocCharOption

character used to display seqloc, such as masked sequence

A generalized representation of a pairwise alignment.

CNcbiOstrstreamToString class helps convert CNcbiOstrstream to a string Sample usage:

CRef< CSeq_align > CreateTranslatedDensegFromNADenseg(void) const

Create a Dense-seg with widths from Dense-seg of nucleotides Used by AlnMgr to handle translated nucl...

CRef< CSeq_align > CreateDensegFromStdseg(SSeqIdChooser *SeqIdChooser=0) const

---------------------------------------------------------------------------- PRE : the Seq-align has ...

TSeqPos GetTotalGapCount(TDim row=-1) const

Retrieves the total number of gaps in the given row an alignment; all gaps by default.

TSeqPos GetSeqStop(TDim row) const

const CSeq_id & GetSeq_id(TDim row) const

Get seq-id (the first one if segments have different ids).

TSeqPos GetSeqStart(TDim row) const

ENa_strand GetSeqStrand(TDim row) const

Get strand (the first one if segments have different strands).

TSeqPos GetAlignLength(bool include_gaps=true) const

Get the length of this alignment.

Base class for all serializable objects.

static void GetBioseqHandleDeflineAndId(const objects::CBioseq_Handle &handle, list< TGi > &use_this_gi, string &seqid, string &defline, bool show_gi=true, TGi this_gi_first=INVALID_GI)

Returns sequence id and a BLAST defline as strings, given a Bioseq handle and a list of gis.

CTypeInfo class contains all information about C++ types (both basic and classes): members and layout...

Interface for filling the top layer of the XML report.

string GetSeqIdString(const CSeq_id &id)

static const char * str(char *buf, int n)

static string GetString(EPublication pub)

Reference for requested publication.

EPublication

Enumerates the various BLAST publications.

@ eMaxPublications

Used as sentinel value.

@ ePhiBlast

1998 NAR paper

@ eCompBasedStats

2001 NAR paper

@ eGappedBlast

1997 NAR paper

@ eMegaBlast

2000 J Compt Biol paper

@ eDeltaBlast

2012 Biology Direct on DeltaBLAST

#define ITERATE(Type, Var, Cont)

ITERATE macro to sequence through container elements.

@ eNoOwnership

No ownership is assumed.

int m_IterationNum

ctor sets to true, set to false for first chunk.

string m_SerialXmlEnd

tag to be printed at end.

static void GetWholeAlnSeqStrings(string &query, string &subject, const objects::CDense_seg &ds, objects::CScope &scope, int master_gen_code, int slave_gen_code)

static string BlastGetVersion(const string program)

Returns the version and release date, e.g.

#define ERR_POST(message)

Error posting with file, line number information but without error codes.

void Error(CExceptionArgs_Base &args)

#define NCBI_THROW(exception_class, err_code, message)

Generic macro to throw an exception, given the exception class, error code and message string.

virtual const char * what(void) const noexcept

Standard report (includes full backlog).

@ eSerialVerifyData_No

do not verify

const string AsFastaString(void) const

CConstRef< CSeq_id > GetSeqId(void) const

bool Match(const CSeq_id &sid2) const

Match() - TRUE if SeqIds are equivalent.

const CSeq_id & GetId(const CSeq_loc &loc, CScope *scope)

If all CSeq_ids embedded in CSeq_loc refer to the same CBioseq, returns the first CSeq_id found,...

TSeqPos GetLength(const CSeq_id &id, CScope *scope)

Get sequence length if scope not null, else return max possible TSeqPos.

@ eGetId_Best

return the "best" gi (uses FindBestScore(), with CSeq_id::CalculateScore() as the score function

CBioseq_Handle GetBioseqHandle(const CSeq_id &id)

Get bioseq handle by seq-id.

TBioseqCore GetBioseqCore(void) const

Get bioseq core structure.

TSeqPos GetBioseqLength(void) const

void Reset(void)

Reset reference object.

int64_t Int8

8-byte (64-bit) signed integer

#define END_NCBI_SCOPE

End previously defined NCBI scope.

#define BEGIN_NCBI_SCOPE

Define ncbi namespace.

IO_PREFIX::ostream CNcbiOstream

Portable alias for ostream.

static string IntToString(int value, TNumToStringFlags flags=0, int base=10)

Convert int to string.

static enable_if< is_arithmetic< TNumeric >::value||is_convertible< TNumeric, Int8 >::value, string >::type NumericToString(TNumeric value, TNumToStringFlags flags=0, int base=10)

Convert numeric value to string.

void SetLambda(TLambda value)

Assign a value to Lambda data member.

void SetExpect(TExpect value)

Assign a value to Expect data member.

void SetGap_open(TGap_open value)

Assign a value to Gap_open data member.

void SetEntropy(TEntropy value)

Assign a value to Entropy data member.

void SetDb_num(TDb_num value)

Assign a value to Db_num data member.

void SetQuery_len(TQuery_len value)

Assign a value to Query_len data member.

void SetHsp_len(THsp_len value)

Assign a value to Hsp_len data member.

void SetSc_match(TSc_match value)

Assign a value to Sc_match data member.

void SetMatrix(const TMatrix &value)

Assign a value to Matrix data member.

void SetProgram(const TProgram &value)

Assign a value to Program data member.

void SetGap_extend(TGap_extend value)

Assign a value to Gap_extend data member.

void SetQuery_ID(const TQuery_ID &value)

Assign a value to Query_ID data member.

void SetVersion(const TVersion &value)

Assign a value to Version data member.

void SetKappa(TKappa value)

Assign a value to Kappa data member.

void SetFilter(const TFilter &value)

Assign a value to Filter data member.

TIterations & SetIterations(void)

Assign a value to Iterations data member.

void SetSc_mismatch(TSc_mismatch value)

Assign a value to Sc_mismatch data member.

void SetDb(const TDb &value)

Assign a value to Db data member.

void SetQuery_def(const TQuery_def &value)

Assign a value to Query_def data member.

void SetParam(TParam &value)

Assign a value to Param data member.

void SetPattern(const TPattern &value)

Assign a value to Pattern data member.

void SetReference(const TReference &value)

Assign a value to Reference data member.

void SetDb_len(TDb_len value)

Assign a value to Db_len data member.

void SetEff_space(TEff_space value)

Assign a value to Eff_space data member.

const TDenseg & GetDenseg(void) const

Get the variant data.

Tdata & Set(void)

Assign a value to data member.

bool IsSetStrands(void) const

Check if a value has been assigned to Strands data member.

void SetSegs(TSegs &value)

Assign a value to Segs data member.

bool IsDendiag(void) const

Check if variant Dendiag is selected.

const TStd & GetStd(void) const

Get the variant data.

const TDendiag & GetDendiag(void) const

Get the variant data.

bool IsStd(void) const

Check if variant Std is selected.

bool IsDisc(void) const

Check if variant Disc is selected.

const TIds & GetIds(void) const

Get the Ids member data.

list< CRef< CSeq_align > > Tdata

const TDisc & GetDisc(void) const

Get the variant data.

const TStrands & GetStrands(void) const

Get the Strands member data.

const Tdata & Get(void) const

Get the member data.

const TSegs & GetSegs(void) const

Get the Segs member data.

bool IsDenseg(void) const

Check if variant Denseg is selected.

static CRef< CSeq_align > CreateDensegFromDendiag(CSeq_align const &aln)

Structure to hold data for incremental XML formatting.


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