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Showing content from http://www.ncbi.nlm.nih.gov/IEB/ToolBox/CPP_DOC/doxyhtml/bam2graph_8cpp_source.html below:

NCBI C++ ToolKit: src/sra/app/bam2graph/bam2graph.cpp Source File

60  virtual void Init

(

void

);

61  virtual int Run

(

void

);

62  virtual void Exit

(

void

);

73 #define BAM_DIR "1000genomes/ftp/data" 74 #define CONFIG_FILE_NAME "analysis.bam.cfg" 82

arg_desc->SetUsageContext(

GetArguments

().GetProgramBasename(),

83  "CArgDescriptions demo program"

);

85

arg_desc->AddOptionalKey(

"srz"

,

"srz"

,

86  "SRZ accession config file" 87  " (-log and -int options are ignored)"

,

89

arg_desc->AddOptionalKey(

"file"

,

"File"

,

92

arg_desc->AddOptionalKey(

"dir"

,

"Dir"

,

93  "BAM files files directory"

,

95

arg_desc->AddOptionalKey(

"odir"

,

"OutputDir"

,

96  "Destination directory"

,

99

arg_desc->AddOptionalKey(

"ref_label"

,

"RefLabel"

,

100  "RefSeq id in BAM file"

,

102

arg_desc->AddOptionalKey(

"seq_id"

,

"SeqId"

,

105

arg_desc->AddOptionalKey(

"delta"

,

"Delta"

,

106  "Delta-ext in text ASN.1"

,

108

arg_desc->AddOptionalKey(

"delta_file"

,

"DeltaFile"

,

109  "File with Delta-ext in text ASN.1"

,

112

arg_desc->AddFlag(

"estimated"

,

"Make estimated graph using index only"

);

113

arg_desc->AddFlag(

"raw-access"

,

"Make graph using raw access to BAM"

);

114

arg_desc->AddOptionalKey(

"min_quality"

,

"MinMapQuality"

,

115  "Minimal alignment map quality"

,

117

arg_desc->AddFlag(

"log"

,

"Generate logarithmic graph"

);

118

arg_desc->AddFlag(

"int"

,

"Generate graph with int values"

);

119

arg_desc->AddOptionalKey(

"max"

,

"OutlierMax"

,

120  "Factor over average to treat as outlier"

,

122

arg_desc->AddFlag(

"max_details"

,

"Include detailed outlier info"

);

123

arg_desc->AddOptionalKey(

"bin_size"

,

"BinSize"

,

124  "Seq-graph bin size"

,

126

arg_desc->AddOptionalKey(

"annot_name"

,

"annot_name"

,

127  "Annot name with generated Seq-graph"

,

129

arg_desc->AddOptionalKey(

"title"

,

"Title"

,

130  "Title of generated Seq-graph"

,

133

arg_desc->AddDefaultKey(

"o"

,

"OutputFile"

,

134  "Output file of ASN.1"

,

137

arg_desc->AddFlag(

"b"

,

"Write binary ASN.1"

);

138

arg_desc->AddFlag(

"annot"

,

"Write Seq-annot only"

);

157  if

( args[

"file"

] ) {

160  else if

( args[

"srz"

] ) {

180  if

( args[

"dir"

] ) {

181

dir = args[

"dir"

].AsString();

186  ITERATE

( vector<string>, it, reps ) {

196  if

( !

CFile

(path).Exists() ) {

199  if

( tt.size() > 0 ) {

208  if

( args[

"ref_label"

] ) {

209

ref_label = args[

"ref_label"

].AsString();

214  if

( tt.size() > 1 ) {

224  if

( args[

"seq_id"

] ) {

225  CSeq_id

seq_id(args[

"seq_id"

].AsString());

232  if

( args[

"annot_name"

] ) {

235  if

( args[

"title"

] ) {

238  if

( args[

"min_quality"

] ) {

241  if

( args[

"log"

] ) {

244  if

( args[

"int"

] ) {

247  if

( args[

"bin_size"

] ) {

250  if

( args[

"max"

] ) {

253  if

( args[

"max_details"

] ) {

256  if

( args[

"estimated"

] ) {

259  if

( args[

"raw-access"

] ) {

263  if

( args[

"delta"

] ) {

265  string

s = args[

"delta"

].AsString();

267

s =

"Delta-ext ::= "

+s;

272  else if

( args[

"delta_file"

] ) {

287  if

( 0 && args[

"estimated"

] ) {

302  CBamDb

bam(mgr, path, path+

".bai"

);

313  if

( args[

"annot"

] ) {

326  if

(

CFile

(srz_name).IsDir() ) {

330  if

( args[

"dir"

] ) {

331

dir = args[

"dir"

].AsString();

337  if

( args[

"odir"

] ) {

338

odir = args[

"odir"

].AsString();

351

vector<string> tokens;

354  while

( getline(srz, line) ) {

357  if

( tokens.size() < 4 ) {

360  string

ref_label = tokens[0];

361  string

acc = tokens[1];

363  if

( tokens[2].

empty

() ) {

369  string

bam_name = tokens[3];

370  if

( tokens.size() < 5 ) {

371  ERR_POST

(

"No coverage requested for "

<<ref_label);

375  string

out_name = tokens[4];

378  LOG_POST

(

"Processing "

<<ref_label<<

" -> "

<<out_path);

383  if

( args[

"bin_size"

] ) {

386  if

( args[

"annot_name"

] ) {

392  if

( args[

"title"

] ) {

398  if

( args[

"min_quality"

] ) {

402  if

( args[

"estimated"

] ) {

405  if

( args[

"raw-access"

] ) {

410  if

( 0 && args[

"estimated"

] ) {

412  if

( bam_path != db_name ) {

414

header.

Read

(bam_path);

415

index.

Read

(bam_path+

".bai"

);

426  if

( bam_path != db_name ) {

429

raw_db =

CBamRawDb

(bam_path, bam_path+

".bai"

);

432

db =

CBamDb

(mgr, bam_path, bam_path+

".bai"

);

464 int main

(

int

argc,

const char

* argv[])

User-defined methods of the data storage class.

int main(int argc, const char *argv[])

virtual void Exit(void)

Cleanup on application exit.

void ProcessFile(const string &file)

void ProcessSrz(string srz_name)

virtual int Run(void)

Run the application.

virtual void Init(void)

Initialize the application.

void SetGraphBinSize(TSeqPos bin_size)

void SetRawAccess(bool raw_access=true)

void SetGraphTitle(const string &title)

bool GetEstimated(void) const

make estimated graph using BAM index only the bin size will be derived from index

void SetOutlierMax(double x)

void SetEstimated(bool estimated=true)

const string & GetAnnotName(void) const

Annot name of generated Seq-graph.

void SetGraphValueType(EGraphValueType type)

void SetAnnotName(const string &name)

void SetOutlierDetails(bool details=true)

CRef< CSeq_entry > MakeSeq_entry(CBamMgr &mgr, const string &bam_file, const string &bam_index)

Generate Seq-entry for BAM file.

void SetRefLabel(const string &ref_label)

void SetGraphType(EGraphType type)

void SetRefId(const CSeq_id &ref_id)

const CSeq_id & GetRefId(void) const

Seq-id for the reference sequence in generated entry.

void SetSeq_inst(CRef< CSeq_inst > inst)

Use specified Seq-inst object for the virtual sequence.

void SetMinMapQuality(int qual)

void Read(const string &index_file_name)

CRef< CSeq_annot > MakeEstimatedCoverageAnnot(const CBamHeader &header, const string &ref_name, const string &seq_id, const string &annot_name, TIndexLevel min_index_level, TIndexLevel max_index_level) const

const TAnnot & GetAnnot(void) const

std::ofstream out("events_result.xml")

main entry point for tests

virtual const CArgs & GetArgs(void) const

Get parsed command line arguments.

int AppMain(int argc, const char *const *argv, const char *const *envp=0, EAppDiagStream diag=eDS_Default, const char *conf=NcbiEmptyCStr, const string &name=NcbiEmptyString)

Main function (entry point) for the NCBI application.

virtual void SetupArgDescriptions(CArgDescriptions *arg_desc)

Setup the command line argument descriptions.

#define ITERATE(Type, Var, Cont)

ITERATE macro to sequence through container elements.

const CNcbiArguments & GetArguments(void) const

Get the application's cached unprocessed command-line arguments.

@ eInputFile

Name of file (must exist and be readable)

@ eDouble

Convertible into a floating point number (double)

@ eString

An arbitrary string.

@ eOutputFile

Name of file (must be writable)

@ eInteger

Convertible into an integer number (int or Int8)

EDiagSev SetDiagPostLevel(EDiagSev post_sev=eDiag_Error)

Set the threshold severity for posting the messages.

#define ERR_POST(message)

Error posting with file, line number information but without error codes.

#define LOG_POST(message)

This macro is deprecated and it's strongly recomended to move in all projects (except tests) to macro...

void SetDiagStream(CNcbiOstream *os, bool quick_flush=true, FDiagCleanup cleanup=0, void *cleanup_data=0, const string &stream_name="")

Set diagnostic stream.

@ eDiag_Info

Informational message.

void Fatal(CExceptionArgs_Base &args)

void Info(CExceptionArgs_Base &args)

string GetDir(EIfEmptyPath mode=eIfEmptyPath_Current) const

Get the directory component for this directory entry.

static string MakePath(const string &dir=kEmptyStr, const string &base=kEmptyStr, const string &ext=kEmptyStr)

Assemble a path from basic components.

#define MSerial_AsnBinary

#define MSerial_AsnText

I/O stream manipulators –.

@ eSerial_AsnBinary

ASN.1 binary.

static CObjectOStream * Open(ESerialDataFormat format, CNcbiOstream &outStream, bool deleteOutStream)

Create serial object writer and attach it to an output stream.

IO_PREFIX::ostream CNcbiOstream

Portable alias for ostream.

IO_PREFIX::ifstream CNcbiIfstream

Portable alias for ifstream.

static list< string > & Split(const CTempString str, const CTempString delim, list< string > &arr, TSplitFlags flags=0, vector< SIZE_TYPE > *token_pos=NULL)

Split a string using specified delimiters.

static bool StartsWith(const CTempString str, const CTempString start, ECase use_case=eCase)

Check if a string starts with a specified prefix value.

double Restart(void)

Return time elapsed since first Start() or last Restart() call (in seconds).

double Elapsed(void) const

Return time elapsed since first Start() or last Restart() call (in seconds).

@ eStart

Start timer immediately after creating.

@ e_Gi

GenInfo Integrated Database.

TSet & SetSet(void)

Select the variant.

TAnnot & SetAnnot(void)

Assign a value to Annot data member.

TSeq_set & SetSeq_set(void)

Assign a value to Seq_set data member.

void SetExt(TExt &value)

Assign a value to Ext data member.

void SetRepr(TRepr value)

Assign a value to Repr data member.

void SetMol(TMol value)

Assign a value to Mol data member.

@ eRepr_delta

sequence made by changes (delta) to others

@ eMol_na

just a nucleic acid

CBioseq_Base_Info & GetBase(CTSE_Info &tse, const CBioObjectId &id)

constexpr bool empty(list< Ts... >) noexcept

Defines the CNcbiApplication and CAppException classes for creating NCBI applications.

Defines classes: CDirEntry, CFile, CDir, CSymLink, CMemoryFile, CFileUtil, CFileLock,...

std::istream & in(std::istream &in_, double &x_)

Int4 delta(size_t dimension_, const Int4 *score_)

Defines location of test data folder at NCBI.

static const char * NCBI_GetTestDataPath(void)

Get the directory where test data is stored at NCBI.


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