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Showing content from http://www.bioconductor.org/packages/devel/bioc/html/../../bioc/html/../../bioc/html/HTqPCR.html below:

Bioconductor - HTqPCR (development version)

HTqPCR

This is the development version of HTqPCR; for the stable release version, see HTqPCR.

Automated analysis of high-throughput qPCR data

Bioconductor version: Development (3.22)

Analysis of Ct values from high throughput quantitative real-time PCR (qPCR) assays across multiple conditions or replicates. The input data can be from spatially-defined formats such ABI TaqMan Low Density Arrays or OpenArray; LightCycler from Roche Applied Science; the CFX plates from Bio-Rad Laboratories; conventional 96- or 384-well plates; or microfluidic devices such as the Dynamic Arrays from Fluidigm Corporation. HTqPCR handles data loading, quality assessment, normalization, visualization and parametric or non-parametric testing for statistical significance in Ct values between features (e.g. genes, microRNAs).

Author: Heidi Dvinge, Paul Bertone

Maintainer: Matthew N. McCall <mccallm at gmail.com>

Citation (from within R, enter citation("HTqPCR")): Installation

To install this package, start R (version "4.5") and enter:


if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

# The following initializes usage of Bioc devel
BiocManager::install(version='devel')

BiocManager::install("HTqPCR")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("HTqPCR")
Details biocViews DataImport, DifferentialExpression, GeneExpression, MicrotitrePlateAssay, MultipleComparison, Preprocessing, QualityControl, Software, Visualization, qPCR Version 1.63.0 In Bioconductor since BioC 2.5 (R-2.10) (15.5 years) License Artistic-2.0 Depends Biobase, RColorBrewer, limma Imports affy, Biobase, gplots, graphics, grDevices, limma, methods, RColorBrewer, stats, stats4, utils System Requirements URL http://www.ebi.ac.uk/bertone/software See More Package Archives

Follow Installation instructions to use this package in your R session.


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