Pysam is a python module for reading and manipulating files in the SAM/BAM format. The SAM/BAM format is a way to store efficiently large numbers of alignments (Li 2009), such as those routinely created by next-generation sequencing methods.
Pysam is a lightweight wrapper of the samtools C-API. Pysam also includes an interface for tabix.
If you are using the conda packaging manager (e.g. miniconda or anaconda), you can install pysam from the bioconda channel:
conda config --add channels bioconda conda config --add channels conda-forge conda config --set channel_priority strict conda install pysam
Installation through bioconda is the recommended way to install pysam as it resolves non-python dependencies and uses pre-configured compilation options. Especially for OS X this will potentially save a lot of trouble.
The current version of pysam wraps 3rd-party code from htslib-1.21, samtools-1.21, and bcftools-1.21.
Pysam is available through PyPI. To install, type:
pip install pysam
Pysam documentation is available here
Questions and comments are very welcome and should be sent to the pysam user group
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